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Parallel Toxicogenomic Resources for Zebrafish and Rainbow Trout: Identifying Conserved Molecular Biomarkers of Toxicant Exposure
This continuing project will use genomic tools to identify toxicant-response molecular biomarkers in zebrafish and rainbow trout for use in assays evaluating the potential effects of exposure to persistant, bioaccumulative toxicants on fish. The investigators will combine DNA microarray work with quantitative reverse transcription-polymerase chain reaction methodologies to identify embryonic and larval genes responsive to dioxin (TCDD), polychlorinated biphenyls (PCBs) and methylmercury (MeHg) exposure. They plan to sequence about 20,000 toxicant-response transcripts for zebrafish and rainbow trout and create publicly accessible, functionally annotated, expressed sequence tag databases—a biomarker library that can be used as reference for examining toxicant responses among Great Lakes wild fish species.
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